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Kumar, Narender
- Extraction of Chitosan and its Oligomers from Shrimp Shell Waste, their Characterization and Antimicrobial Effect
Authors
1 Department of Animal Nutrition, ICAR-National Dairy Research Institute, Karnal, Haryana, IN
2 Department of Animal Nutrition, ICAR-National Institute of Animal Nutrition and Physiology, Adugodi, Bengaluru, Karnataka, IN
3 ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, IN
Source
Veterinary World, Vol 10, No 2 (2017), Pagination: 170-175Abstract
Aim: The present study was performed to utilize the shrimp shell waste for chitin and chitosan production, characterization by Fourier transform infrared (FT-IR) technique and to evaluate the antimicrobial effects of chitosan oligomers produced by depolymerization of chitosan by nitrous acid.
Materials and Methods: Chitosan was extracted from the shrimp shell waste by the chemical method and characterized by FT-IR. Chitooligomers were produced by depolymerising chitosan using nitrous acid, and the chitooligomers were tested for antimicrobial effect against four gut pathogenic organisms, i.e., Enterobacter aerogen (National Collection of Dairy Culture [NCDC] 106), Enterococcus faecalis (NCDC 119), Escherichia coli (NCDC 134), and Staphylococcus aureus (NCDC 109) by well diffusion method using Muller-Hinton agar. A pure culture of pathogenic organisms was collected from NCDC, ICAR-National Dairy Research Institute, Karnal.
Results: Extracted chitosan characterized by FT-IR and chitooligomers demonstrated antimicrobial effect against four gut pathogenic organisms used in this study. Zone of inhibitions (mm) were observed in E. faecalis (13±0.20), E. coli (11.5±0.4), S. aureus (10.7±0.2), and E. aerogen (10.7±0.3). E. faecalis showed larger inhibition zone as compared to all other organisms and inhibitions zones of E. aerogen and S. aureus were comparable to each other.
Conclusion: Shrimp waste can be utilized for chitosan production, and the chitooligomers can be used as feed additive for gut health enhancement and have potential to replace antibiotics from the feed. Along with value addition pollutant load could be reduced by waste utilization.
Keywords
Chitin, Chitooligomers, Chitosan, Fourier Transform Infrared, Shrimp Waste.- Influence of Clinical Mastitis and its Treatment Outcome on Reproductive Performance in Crossbred Cows:A Retrospective Study
Authors
1 Livestock Research Centre, Southern Regional Station, ICAR-National Dairy Research Institute (NDRI), Adugodi, Bengaluru-560030, Karnataka, IN
2 Dairy Economics and Statistics Section, Southern Regional Station, ICAR-National Dairy Research Institute (NDRI), Adugodi, Bengaluru-560030, Karnataka, IN
3 Theriogenology Laboratory, Animal Reproduction, Gynaecology & Obstetrics, Southern Regional Station, ICAR-National Dairy Research Institute (NDRI), Adugodi, Bengaluru-560030, Karnataka, IN
4 Livestock Production and Management Section, ICAR National Dairy Research Institute (NDRI), Karnal-132001, Haryana, IN
5 ICAR-National Institute of Biotic Stress Management, Baronda, Raipur-493225, Chhattisgarh, IN
6 Division of Animal Nutrition, ICAR-National Institute of Animal Nutrition and Physiology, Adugodi, Bengaluru-560030, Karnataka, IN
Source
Veterinary World, Vol 10, No 5 (2017), Pagination: 485-492Abstract
Aim: Evaluation of the effect of clinical mastitis (CM) and its treatment outcome on the reproductive performance in crossbred cows retrospectively.Materials and Methods: Datasets of 835 lactating cows affected with CM during a period of 12 years (2001-2012) were considered for this study. Mastitis treatment related data and reproductive parameters such as days to first detected heat (DTFDH), days to first insemination (DTFI), days open (DO), and number of services per conception (SC) were collected from mastitis treatment and artificial insemination registers, respectively. Data were analyzed by ANOVA using SPSS 20 software. The means were compared with the Duncan’s multiple comparison post-hoc test.
Results: CM affected cows had significantly (p<0.05) higher DTFDH, DTFI, DO and SC compared to clinically healthy cows. Cows diagnosed with a single episode of CM had significantly (p<0.05) delayed DTFDH while, DO and SC were significantly higher (p<0.05) in cows diagnosed by multiple episodes of CM. SC was significantly (p<0.05) higher in cows diagnosed with both relapse and recurrence. Severe CM affected cows had significantly (p<0.05) altered reproductive parameters. The reproductive parameters were altered to high extent when CM occurred during the breeding period.
Conclusion: CM-affected cows had higher DTFDH, DTFI, DO and SC compared to clinically healthy cows. The negative effects of CM on reproduction parameters were higher when CM occurred during the breeding period.